研究開発・学会発表

2月のおはな

Fertility and Sterility

学会名

Fertility and Sterility

日・場所

Vol.90,No.5,Novenber 2008(平成20年11月)

タイトル

Fertilization and pregnancy using cryopreserved testicular sperm for intracytoplasmic sperm injection with azoospermia

発表者名

Tomomoto Ishikawa M.D., Ph.D.a, Masahide Shiotani M.D., Ph.D.b, Yoko Izumi H.C.L.D.b, Hiromi Hashimoto H.C.L.D.b, Shoji Kokeguchi, M.D., Ph.D.b, Sakae Goto, M.D., Ph.D. b, Masato Fujisawa, M.D., Ph.D.a
a Division of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe, Japan
b Hanabusa Women’s Clinic, Kobe, Japan

【要旨】

Objectives:Fertilization and pregnancies can be obtained with spermatozoa recovered from the seminiferous tubules.Wehereby report fertility for men with obstructive (OA) and non-obstructive azoospermia (NOA) patients following treatment by frozen and thawed testicular spermatozoa (cryoTESE)-ICSI and evaluate the factors other than spermatozoa.
Design:Retrospective clinical analysis.
Setting:Male infertility clinic for TESE and ART clinic for ICSI.
Patients: Seventy-four men with OA and 140 men with NOA undergoing attempted cryoTESE-ICSI.
Interventions:Seventy-three couples with OA underwent a total of 184 cryoTESE-ICSI and 46 couples with NOA underwent a total of 75 cryoTESE-ICSI treatmentcycles.
Main outcome measure(s): The number of eggs at MII injected, 2 pronuclei (PN) oocytes, normal cleaved embryos, embryos transferred, transfers cycles, biochemical pregnancies, clinical pregnancies, implantation rate, and delivered were examined.
Results: Fertilization rate in NOA was significantly less common than in OA. Neither the pathology, the source, nor the quantity of spermatozoa had any effect on fertilization or pregnancy rates. Maternal age had no effect on fertilization or embryo cleavage, but did dramatically affect the implantation, pregnancy and delivery rates both.
Conclusions:Good pregnancy rates were achieved without significant differences among the sperm sources. The pregnancy and the delivery rate were dependent strictly on the age of the wife but not on her ovarian reserve.